Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Yellow 690, eBioscience

Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (IT2.2), NovaFluor Yellow 690, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 647, 700
• APC, Biotin
• Brilliant UV 737, Brilliant Violet 480, 711, 786
• Functional Grade
• NovaFluor Blue 660-120S, Red 700, 725, 755
• NovaFluor Yellow 610, 700, 755
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Request custom conjugation

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Product Specific Information

The IT2.2 monoclonal antibody reacts with human CD86 (B7-2), an ~80 kDa surface receptor. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated by stimuli such as BCR complex, CD40, and cytokine receptors.
CD86 interacts with T cell surface molecules CD28 and CTLA-4, playing a critical role in T-B crosstalk, T cell costimulation, and antibody production.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry on human peripheral blood cells (5 µL per test, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow)

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Target Information

CD86 is a ligand for CTLA4 and CD28, acting as a costimulatory molecule during antigen presentation. Insufficient co-stimulation can induce tolerance. CD86 is a type I membrane protein in the immunoglobulin superfamily, expressed by antigen-presenting cells.
Binding with CD28 activates T cells, while binding with CTLA4 suppresses activation and immune response.
Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with gallbladder squamous cell carcinoma and myocarditis.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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