Thermo Fisher Scientific PDHA1 Polyclonal Antibody
Thermo Fisher Scientific의 PDHA1 Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 PDHA1 단백질 검출용으로 검증된 항체입니다. Western blot, IHC, ICC/IF, ELISA에 적합하며 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 30–390 of human PDHA1 (NP_0002751) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.718 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855501 |
Product Specific Information
Immunogen sequence:
FANDATFEIK KCDLHRLEEG PPVTTVLTRE DGLKYYRMMQ TVRRMELKAD QLYKQKIIRG FCHLCDGQEA CCVGLEAGIN PTDHLITAYR AHGFTFTRGL SVREILAELT GRKGGCAKGK GGSMHMYAKN FYGGNGIVGA QVPLGAGIAL ACKYNGKDEV CLTLYGDGAA NQGQIFEAYN MAALWKLPCI FICENNRYGM GTSVERAAAS TDYYKRGDFI PGLRVDGMDI LCVREATRFA AAYCRSGKGP ILMELQTYRY HGHSMSDPGV SYRTREEIQE VRSKSDPIML LKDRMVNSNL ASVEELKEID VEVRKEIEDA AQFATADPEP PLEELGYHIY SSDPPFEVRG ANQWIKFKSV S
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle.
The PDH complex consists of multiple copies of three enzymatic components:
- E1: Pyruvate dehydrogenase
- E2: Dihydrolipoamide acetyltransferase
- E3: Lipoamide dehydrogenase
The E1 enzyme is a heterotetramer composed of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit, which contains the E1 active site and plays a key role in PDH complex function.
Mutations in PDHA1 are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternatively spliced transcript variants encoding different isoforms have been identified.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-110090_PDHA1_P08559-1_Rabbit.svg, PA5-110090_PDHA1_P08559-1_Rabbit_PDP.jpeg)
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