Thermo Fisher Scientific PDK4 Polyclonal Antibody
Thermo Fisher Scientific의 PDK4 Polyclonal Antibody는 인간, 생쥐, 랫트 반응성을 가지며 Western blot, IHC, ICC, ELISA 등에 사용 가능합니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 102–411 of human PDK4 (NP_002603.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.35 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855503 |
Product Specific Information
Immunogen sequence:
FHEKSPDDQK ALSDFVDTLI KVRNRHHNVV PTMAQGIIEY KDACTVDPVT NQNLQYFLDR FYMNRISTRM LMNQHILIFS DSQTGNPSHI GSIDPNCDVV AVVQDAFECS RMLCDQYYLS SPELKLTQVN GKFPDQPIHI VYVPSHLHHM LFELFKNAMR ATVEHQENQP SLTPIEVIVV LGKEDLTIKI SDRGGGVPLR IIDRLFSYTY STAPTPVMDN SRNAPLAGFG YGLPISRLYA KYFQGDLNLY SLSGYGTDAI IYLKALSSES IEKLPVFNKS AFKHYQMSSE ADDWCIPSRE PKNLAKEVAM
Target Information
PDK4 (PDHK4), along with other PDHKs, regulates glucose metabolism by phosphorylating the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex. Inhibition of PDHKs is viewed as a potential therapeutic approach for type II diabetes. This protein is located in the mitochondrial matrix, and its expression is regulated by glucocorticoids, retinoic acid, and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex via phosphorylation of the E1 alpha subunit, contributing to glucose metabolism regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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