Thermo Fisher Scientific C/EBP alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant rat C/EBP alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2078043

Product Specific Information

PA1-825 detects C/EBP alpha protein in mouse samples.

PA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 30 and 43 kDa protein representing C/EBP alpha in mouse 3T3-L1 cells. These polypeptides are derived from alternative translation initiation.

Target Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.