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Takara Stem Cell Pluripotency Status Check Kit with PCR Primers
제품 개요
The Pluripotency Check PCR Kit contains all necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent.
It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes.
Different amplicon sizes allow distinction between endogenous and exogenous expression.
The kit provides everything for start-to-finish confirmation of your cells’ pluripotency status using any combination of primer sets or the entire panel.
주요 특징
- Validate the pluripotent status of ES or iPS cell cultures using a simple PCR kit
- 16 prevalidated PCR primer mixes
- Complete kit with RNA extraction, reverse transcription, and PCR reagents
- Confirm iPS cells by differentiating between endogenous and exogenous gene expression
Applications
- Validating mouse embryonic stem cell status
- Confirming iPS status
Pluripotency Check PCR Primers
| Primer Set | Amplicon Size | Primer Set | Amplicon Size |
|---|---|---|---|
| Oct3/4 (Total) | 485 bp | Klf4 | 739 bp |
| Oct3/4 (Endogenous) | 224 bp | Klf4 (Endogenous) | 206 bp |
| Nanog (Total) | 364 bp | Ecat1 | 164 bp |
| Nanog (Endogenous) | 223 bp | ERas1 | 210 bp |
| Sox2 (Total) | 193 bp | Esg1 | 376 bp |
| Sox2 (Endogenous) | 297 bp | Rex1 | 287 bp |
| c-Myc (Total) | 228 bp | Beta-Actin (Control) | 603 bp |
| c-Myc (Endogenous) | 170 bp | GAPDH (Control) | 568 bp |
추가 정보
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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