
Takara Stem cell pluripotency status check kit with PCR pimers
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The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The different amplicon sizes allow you to distinguish between endogenous and exogenous expression.
The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The different amplicon sizes allow you to distinguish between endogenous and exogenous expression. The kit includes everything for start-to-finish confirmation of your cells’ pluripotency status, using any combination of primer sets, or the entire panel.
Overview
- Validate the pluripotent status of your ES or iPS cell culture using a simple PCR kit
16 prevalidated PCR primer mixes
Complete kit with RNA extraction, reverse transcription, and PCR reagents
Confirm your iPS cells by differentiating between endogenous and exogenous gene expression
More Information
Applications
Validating mouse embryonic stem cell status
Confirming iPS status
Pluripotency check PCR primers
Primer set | Amplicon size | Primer set | Amplicon size |
Oct3/4 (Total) | 485 bp | Klf4 | 739 bp |
Oct3/4 (Endogenous) | 224 bp | Klf4 (Endogenous) | 206 bp |
Nanog (Total) | 364 bp | Ecat1 | 164 bp |
Nanog (Endogenous) | 223 bp | ERas1 | 210 bp |
Sox2 (Total) | 193 bp | Esg1 | 376 bp |
Sox2 (Endogenous) | 297 bp | Rex1 | 287 bp |
c-Myc (Total) | 228 bp | Beta-Actin (Control) | 603 bp |
c-Myc (Endogenous) | 170 bp | GAPDH (Control) | 568 bp |
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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