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Takara Stem cell pluripotency status check kit with PCR pimers
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Takara Stem cell pluripotency status check kit with PCR pimers

상품 한눈에 보기

마우스 ES 및 iPS 세포의 다능성 상태를 간편하게 확인하는 PCR 키트. 16종의 검증된 프라이머 믹스 포함. 내인성 및 외인성 유전자 발현 구분 가능. RNA 추출부터 PCR까지 전 과정에 필요한 시약 포함.

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Takara
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PCR Kits
판매단위
pk
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Takara 631965 Pluripotency Check PCR Kit, Each pk판매 단위 pk ·
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Takara 631966 Pluripotency Check PCR Primer Set, 16 x 25 Rxns pk판매 단위 pk ·
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Takara · Takara Stem cell pluripotency status check kit with PCR pimers

Takara Stem Cell Pluripotency Status Check Kit with PCR Primers

제품 개요

The Pluripotency Check PCR Kit contains all necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent.
It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes.
Different amplicon sizes allow distinction between endogenous and exogenous expression.
The kit provides everything for start-to-finish confirmation of your cells’ pluripotency status using any combination of primer sets or the entire panel.

주요 특징

  • Validate the pluripotent status of ES or iPS cell cultures using a simple PCR kit
  • 16 prevalidated PCR primer mixes
  • Complete kit with RNA extraction, reverse transcription, and PCR reagents
  • Confirm iPS cells by differentiating between endogenous and exogenous gene expression

Applications

  • Validating mouse embryonic stem cell status
  • Confirming iPS status

Pluripotency Check PCR Primers

Primer Set Amplicon Size Primer Set Amplicon Size
Oct3/4 (Total) 485 bp Klf4 739 bp
Oct3/4 (Endogenous) 224 bp Klf4 (Endogenous) 206 bp
Nanog (Total) 364 bp Ecat1 164 bp
Nanog (Endogenous) 223 bp ERas1 210 bp
Sox2 (Total) 193 bp Esg1 376 bp
Sox2 (Endogenous) 297 bp Rex1 287 bp
c-Myc (Total) 228 bp Beta-Actin (Control) 603 bp
c-Myc (Endogenous) 170 bp GAPDH (Control) 568 bp

추가 정보

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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