Takara Bicistronic IRES Tet-On 3G inducible expression systems

Takara Bicistronic IRES Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems expresses two transgenes from the premium TRE3G inducible promoter (PTRE3G).

You can either:

• Co-express two different genes of interest using systems that contain the pTRE3G-IRES Vector, which includes two multiple cloning sites flanking an IRES sequence (Cat. #631166 and #631346), or
• Monitor co-induced expression of your transgene with a fluorescent protein using kits that contain pTRE3G-mCherry (Cat. #631165 and #631347) or pTRE3G-ZsGreen1 (Cat. #631164 and #631348).

These IRES-containing bicistronic vectors allow the simultaneous induced expression of two proteins separately but from the same RNA transcript.

How Does an IRES Work?

The Internal Ribosome Entry Site (IRES) of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from a single messenger RNA.
Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5′ cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location.
Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript.

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