Thermo Fisher Scientific CD20 Recombinant Rabbit Monoclonal Antibody (6L3K5)
Thermo Fisher의 CD20 Recombinant Rabbit Monoclonal Antibody (Clone 6L3K5)는 인간 CD20 단백질을 인식하는 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 고순도 Affinity chromatography 정제, 안정적 PBS/glycerol buffer 보존, 연구용으로 최적화된 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:800–1:3,200 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6L3K5 |
| Immunogen | A synthetic peptide corresponding to amino acids 100–200 of human CD20 (NP_068769.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.04 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2898042 |
Product Specific Information
- Positive Samples: A-549, Raji, U-937
- Immunogen Sequence:
LLAATEKNSR KCLVKGKMIM NSLSLFAAIS GMILSIMDIL NIKISHFLKM ESLNFIRAHT PYINIYNCEP ANPSEKNSPS TQYCYSIQSL FLGILSVMLI F
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. It regulates transmembrane calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts, and its expression correlates with surface IgM. It remains on the plasma membrane after antibody treatment, making it a key therapeutic target in B-cell malignancies.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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