Thermo Fisher Scientific Phosphothreonine Recombinant Rabbit Monoclonal Antibody (RM102)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Immunoprecipitation (IP)

1:100-1:500

-

ChIP assay (ChIP)

1:100-1:500

-

Luminex (LUM)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM102

Immunogen

Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2744991

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. There are three amino acids that are typically phosphorylated in eukaryotes: serine, threonine, and tyrosine. Threonine residues in endogenous proteins undergo phosphorylation through the action of a threonine kinase. Small amounts of free phosphothreonine can be detected in urine Antibodies against phosphothreonine can be used as an identifier in the analysis of peptides, proteins, and enzymes. [National Center for Biotechnology Information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.