Thermo Fisher Scientific Phosphothreonine Recombinant Rabbit Monoclonal Antibody (RM102)
Phosphothreonine에 특이적인 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 분석에 활용 가능. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 연구용으로만 사용.
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Thermo Fisher Scientific Phosphothreonine Recombinant Rabbit Monoclonal Antibody (RM102)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | 1:100–1:500 |
| Luminex (LUM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM102 |
| Immunogen | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine-containing peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744991 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Phosphothreonine is a phosphoamino acid, the phosphorylated ester of threonine. It is one of three amino acids typically phosphorylated in eukaryotes—serine, threonine, and tyrosine.
Threonine residues undergo phosphorylation through threonine kinase activity. Small amounts of free phosphothreonine may be detected in urine.
Antibodies against phosphothreonine are useful for identifying peptides, proteins, and enzymes in biochemical analyses.
(Source: National Center for Biotechnology Information)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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