Thermo Fisher Scientific p27Kip1 Recombinant Rabbit Monoclonal Antibody (RM302)
Recombinant rabbit monoclonal antibody recognizing p27Kip1 with high specificity and sensitivity. Validated for WB and IHC (Paraffin). Provides lot-to-lot consistency and animal origin-free formulation. Ideal for cell cycle regulation and TGFβ pathway ...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM302 |
| Immunogen | A peptide corresponding to the C-terminus of human p27Kip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744988 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the optimal candidates for production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and is proposed to mediate TGFβ-induced G1 arrest. It is a key regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms complexes with cyclin D-CDK4, influencing assembly, stability, and activation of CCND1-CDK4 complexes. Its activity depends on phosphorylation state and stoichiometry. p27 Kip1 (~27 kDa) degradation, triggered by CDK-dependent phosphorylation and ubiquitination by SCF complexes, is essential for the transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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