
Thermo Fisher Scientific Rabbit anti-Human Lambda Light Chain Recombinant Secondary Antibody, Biotin
Recombinant rabbit monoclonal secondary antibody conjugated with biotin for human lambda light chain detection. Provides high specificity, sensitivity, and lot-to-lot consistency. Suitable for IHC, ICC/IF, and ELISA applications. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.05–0.2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM127 |
| Immunogen | Human IgG |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744983 |
| Target | Lambda light chain |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified reagents with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies provide:
- Increased versatility across detection systems (HRP, AP, fluorescence)
- Greater sensitivity via signal amplification
Typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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