Thermo Fisher Scientific NSE Monoclonal Antibody (5E2)

Thermo Fisher Scientific NSE Monoclonal Antibody (5E2)

Applications

• Immunohistochemistry (Paraffin) (IHC (P))

Tested Dilution: Ready-to-use 150–200 µL

Product Specifications

Product Specific Information

This product is diluted and in a ready-to-use formulation.
A recommended positive control tissue for this product is cerebellum, though positive controls are not limited to this tissue type.

The antibody is intended for professional laboratory use in detecting the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. It should be used after primary diagnosis of tumor via conventional histopathology using non-immunological stains.

Neuron-specific enolase (NSE) is a glycolytic isoenzyme of the enolase gamma-gamma dimer detected in neurons and neuroendocrine cells, as well as their tumors. NSE is also found in non-neoplastic cells of pituitary, peptide-secreting tissues, pineocytes, neuroendocrine cells of lung and thyroid, parafollicular cells, adrenal medulla, islets of Langerhans, Merkel cells, and melanocytes. Anti-NSE immunostaining is positive in normal striated muscle, hepatocytes, and to a lesser extent, smooth muscle. It serves as a useful marker for peripheral nerves.

For neuroendocrine differentiation identification, it should be used in a panel with more specific markers such as anti-synaptophysin, anti-chromogranin, and anti-neurofilament.

Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. IHC staining techniques visualize antigens via sequential application of primary antibody, secondary antibody, enzyme complex, and chromogenic substrate with washing steps. Results are interpreted under a light microscope to aid in differential diagnosis.

A positive tissue control must be included in each staining procedure. This control should contain both positive and negative staining cells or components. External positive control materials should be fresh specimens processed similarly to patient samples. Tissues with weak positive staining are ideal for detecting minor reagent degradation or sensitivity changes.

Negative control tissue (internal or external) should demonstrate absence of specific staining to confirm lack of non-specific background. If staining occurs in negative control sites, patient results must be considered invalid.

Target Information

Neuron-specific enolase (NSE, ENO1, ENO2, ENO3) catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse in gluconeogenesis. NSE is stable in biological fluids and diffuses into extracellular medium and CSF when neuronal membranes are injured.

NSE is one of three mammalian enolases (alpha, beta, gamma). Alpha is expressed in most tissues, beta only in muscle, and gamma primarily in neurons and neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineage, and neuroendocrine cells. NSE release from damaged neurons serves as a biomarker of neuronal injury in disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also observed in plasma and certain neoplasias including pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.