Thermo Fisher Scientific ARL3 Monoclonal Antibody (OTI2G7), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily.
The 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARL13 and 43% identity with human ARF1.
Like ARFs, ARL3 has a glycine at position 2 (site of N-myristoylation) and lacks cysteine residues near the C terminus, differentiating it from other RAS family members.

Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis.
A 5.5-kb transcript was also detected in most tissues, with highest expression in brain.
Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells.
Although ARL3 binds GTP, it lacks activity in cholera toxin-dependent ADP-ribosylation of Gs, thus classified as an ARF-like protein.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.