Thermo Fisher Scientific ARL3 Monoclonal Antibody (OTI3E1), TrueMAB

Applications

Tested Application & Dilution

• Western Blot (WB): 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily.
The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARL13 and 43% identity with human ARF1.
Like ARFs, ARL3 has a glycine at position 2 (site of N-myristoylation) and lacks cysteine residues near the C terminus found in other RAS family members.
Expression analysis shows ARL3 transcripts in various tissues, highest in heart and lung, lower in brain, liver, kidney, ovary, and testis.
Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells.
Although ARL3 binds GTP, it lacks cholera toxin-dependent ADP-ribosylation activity of Gs, classifying it as an ARF-like protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.