Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), Super Bright 436, eBioscience
마우스 CD140a(PDGFRA) 인식 단클론 항체로, Super Bright 436 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD140a (PDGFRA) Monoclonal Antibody (APA5), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 25 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | APA5 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm (View spectra) |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2662718 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRα is a receptor tyrosine kinase that forms dimers upon ligand binding, activating phosphorylation of substrates. Dimers can be alpha/alpha, beta/beta, or alpha/beta combinations, serving as substrates for kinase activity. CD140a is expressed in embryonic tissues and mesenchymal-derived adult mouse cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of NIH/3T3 cells. Recommended ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize by titration for best performance.
Fluorochrome Information:
Super Bright 436 is excited with a 405 nm violet laser and emits at 436 nm. Recommended detection using a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use the Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
PDGFRA (platelet-derived growth factor receptor alpha) is a cell surface receptor tyrosine kinase that binds PDGF family members, which are mitogens for mesenchymal-origin cells. The receptor forms homo- or heterodimers (alpha/alpha or alpha/beta), depending on the bound ligand. Knockout mouse studies show PDGFRA is essential for kidney development, as heterozygous mice exhibit kidney defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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