Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright 436, eBioscience
Mouse CD140b (PDGFRB) 단백질을 인식하는 APB5 클론 단일클론 항체로, Super Bright 436 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 세포 분석 및 형광 이미징 연구용으로 적합합니다.
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- 62-1402-8x (2개 옵션)
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Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 1.0 µg/test | View 7 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | APB5 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744789 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 600
- Super Bright 780
- Custom conjugation available
Product Specific Information
The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet-derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers upon ligand binding and phosphorylates substrates. Dimers may be alpha/alpha, beta/beta, or alpha/beta combinations, serving as substrates for kinase activity.
CD140b is expressed in embryonic tissues and mesenchymal-derived cells of adult mouse tissues. PDGFR beta plays a significant role in fibrous atherosclerotic lesion formation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on NIH/3T3 cells at ≤1.0 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Super Bright 436 is excited at 405 nm (violet laser) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
PDGFRB is a receptor tyrosine kinase implicated in atherogenesis and cell transformation by the SIS oncogene. These growth factors act as mitogens for mesenchymal-origin cells. The growth factor bound determines whether the receptor forms alpha/alpha, beta/beta, or alpha/beta dimers. The PDGFRB gene is located on chromosome 5 near granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor genes, all associated with 5-q syndrome. A translocation between chromosomes 5 and 12 involving PDGFRB and ETV6 leads to chronic myeloproliferative disorder with eosinophilia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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