Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (APO-1-1), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

APO-1-1

Immunogen

Rec. Human CD95 (APO-1/Fas)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598798

Product Specific Information

Description: Immunohistochemistry:

APO-1-1 (BMS151, IgG1) is an isotype-switch variant of the original clone APO-1-3 (IgG3). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, BMS151 is especially useful for immunohistology on cryostat sections and on cytospin preparations.

Flow cytometry:

BMS151 is also suitable as primary antibody in staining for FACS analysis, BMS151FI can be used for direct staining of cells, BMS151BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Recommended concentration for the unlabelled antibody is 1 - 2 µg/mL and a 1:20 dilution of the labelled antibodies, as determined on SKW6.4 cells.

Applications Tested: Flow Cytometry, Immunohistochemistry.

Purity: >95%.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.