Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), APC, eBioscience

Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), APC, eBioscience

Applications

• Immunohistochemistry (Frozen) (IHC (F))

  • Tested Dilution: -
  • View 2 publications

• Flow Cytometry (Flow)

  • Tested Dilution: 5 µL (0.5 µg)/test
  • View 17 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 650 / 786
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 660-120S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 590 / 660 / 755
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Request custom conjugation

Product Specific Information

Description:
The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Crossreactivity is also seen in baboon, rhesus, and cynomolgus monkey.

Applications Reported:
This W6/32 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.5 µg) per test.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number can range from 10⁵ to 10⁸ cells/test.

Excitation / Emission:
633–647 nm / 660 nm (Red Laser)

Filtration:
0.2 µm post-manufacturing filtered

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus critical for anti-viral and anti-tumor defense. Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.

HLA and MHC antibodies play a key role in Immunopeptidomics, facilitating identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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