Thermo Fisher Scientific Lambda light chain Monoclonal Antibody (1-155-2), APC, eBioscience
인간 Lambda light chain을 인식하는 단클론 항체로, APC로 결합되어 유세포분석(Flow Cytometry)에 적합합니다. 1-155-2 클론이며, 633–647 nm에서 여기, 660 nm에서 방출됩니다. 친화 크로마토그래피로 정제되었으며 4°C 암소 보관이 권장됩니다.
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Thermo Fisher Scientific Lambda light chain Monoclonal Antibody (1-155-2), APC, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested dilution: 5 µL (0.25 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1-155-2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043868 |
Available Formats
Product Specific Information
The monoclonal antibody 1-155-2 recognizes the lambda light chain of human immunoglobulin. The Ig lambda chain (~22 kDa) consists of one variable and one constant region. Two lambda chains, together with heavy chains, form the complete immunoglobulin structure. Expression of Ig lambda is typically found in less than half of B lymphocytes and has been associated with neoplastic lymphomas.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells.
- Recommended use: 5 µL (0.25 µg) per test (100 µL final volume)
- Cell number: 10⁵–10⁸ cells/test (empirically determined)
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Immunoglobulins share a basic structure of four polypeptide chains: two heavy and two light chains (kappa or lambda, ~22.5 kDa). Kappa and lambda chains each have variable and constant regions, distinguishable by their antigenic properties. The kappa:lambda ratio is approximately 70:30.
Antibodies to the kappa light chain are useful in identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates suggests malignancy.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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