Thermo Fisher Scientific Phospho-AIF (Ser116) Polyclonal Antibody
Phospho-AIF(Ser116)을 인식하는 Rabbit Polyclonal 항체로, Western blot에 최적화됨. 인산화된 AIFM1 단백질 검출에 사용. 항원 친화 크로마토그래피로 정제됨. 인산화 상태 특이적 분석 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-AIFM1 (Ser-116) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-116 in human AIFM1. This site is conserved in rat and mouse AIFM1, but the amino acids surrounding the site are not well conserved. The site is not found in AIFM2 and AIFM3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942873 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide.
It detects a triplet at 66, 62, and 57 kDa protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A.
This reactivity is not observed after lambda phosphatase treatment, indicating specificity for phosphorylated AIFM1.
Target Information
Apoptosis Inducing Factor (AIF) is involved in chromatin condensation and DNA fragmentation during apoptosis.
AIF localizes in mitochondria and translocates to the nucleus upon apoptotic stimulation, promoting the release of cytochrome c and caspase-9.
It induces chromatin condensation and large-scale DNA fragmentation, key features of apoptosis.
AIF is highly conserved between human and mouse and is widely expressed.
Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy.
Alternative splicing results in multiple transcript variants, and a related pseudogene is identified on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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