Thermo Fisher Scientific Arp2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Arp2 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp2. This peptide sequence is highly conserved in rat, mouse, chicken, and frog Arp2 proteins.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942877

Product Specific Information

The antibody detects 44 and 32 kDa* proteins corresponding to the molecular mass of Arp2 on SDS-PAGE immunoblots of human A431, HUVEC, HeLa, and Jurkat cells as well as rat PC12, mouse C2C12, and rabbit fibroblasts.

Target Information

ARP2 (actin-related protein 2) is an ATP-binding component of the ARP2/3 complex - a multiprotein complex that mediates actin polymerization upon stimulation by the nucleation-promoting factor (NPF). The ARP2/3 complex mediates the formation of branched actin networks in the cytoplasm and provides the force for cell motility. The ARP2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.