Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 610-30S, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Blue 610-30S

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.4 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow variants
• PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. Together with CD21, CD81, and MHC class II, CD19 forms a multimolecular complex associated with the B cell receptor (BCR).
Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes; ≤0.4 µg/test recommended
• A test is defined as staining a cell sample (100 µL volume, 10^5–10^8 cells/test).
• Antibody should be titrated for optimal performance.

Note: NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.

Each NovaFluor conjugate or kit includes CellBlox Blocking Buffer.

• Add 5 µL per sample when staining with NovaFluor conjugates.
• For single-color controls, use 5 µL per 100 µL cell sample (10^3–10^8 cells).

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures engineered for minimal spillover and spread.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains.
It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells.
CD19 serves as a signal-amplifying co-receptor for the B cell receptor and regulates normal and malignant B cell proliferation.
Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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