Thermo Fisher Scientific PDIA3 Monoclonal Antibody (OTI3H5), TrueMAB
PDIA3 단백질을 인식하는 Mouse monoclonal 항체로 Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, 동결건조 형태로 제공. 1 mg/mL 농도, PBS buffer(8% trehalose) 구성. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 140–505 of human PDIA3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The PDIA3 gene encodes an endoplasmic reticulum protein that interacts with lectin chaperones calreticulin and calnexin to assist in folding newly synthesized glycoproteins. Although previously thought to be a phospholipase, it has been shown to possess protein disulfide isomerase activity. Complexes of lectins and PDIA3 facilitate correct protein folding by promoting disulfide bond formation in glycoprotein substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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