Thermo Fisher Scientific UBE2J1 Monoclonal Antibody (OTI3D5), TrueMAB
Human UBE2J1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC에 적합. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 함유. 연구용으로 단백질 유비퀴틴화 경로 분석에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D5 |
| Immunogen | Full length human recombinant protein of human UBE2J1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The modification of proteins with ubiquitin is a key cellular mechanism for degrading abnormal or short-lived proteins. Ubiquitination involves three enzyme classes: E1 (activating), E2 (conjugating), and E3 (ligase).
UBE2J1 encodes an E2 ubiquitin-conjugating enzyme located in the endoplasmic reticulum membrane, contributing to ER-associated degradation via the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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