Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), eBioscience
인간 CD80(B7-1)을 인식하는 2D10.4 클론 단클론 항체로, Flow Cytometry 등에 사용 가능. 활성화된 B세포, 대식세포, 수지상세포에서 발현되는 CD80 분석에 적합. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4°C 보관.
- 카탈로그번호
- 14-0809-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 3 publications |
| Flow Cytometry (Flow) | 1 µg/test | 19 publications |
| Functional Assay (Functional) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D10.4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467350 |
Available Conjugates
- APC
- Biotin
- Brilliant UV 395 / 615 / 661 / 737
- Brilliant Violet 605 / 650 / 711
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Request custom conjugation available
Product Specific Information
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages, dendritic cells, and activated T cells. It binds with high affinity to T cell surface antigens CD28 and CD152 (CTLA-4), playing a crucial role in T-B cell communication and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells. Recommended ≤1 µg/test in 100 µL final volume. Optimal titration is advised per assay.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of the T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells, essential for T cell activation, and can substitute for each other.
CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes.
It provides regulatory signals for T lymphocytes via CD28 and CTLA4 binding.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.