Thermo Fisher Scientific CD83 Monoclonal Antibody (Michel-17 (Michel17)), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Biotin
• eFluor 660
• FITC
• PE
• Request custom conjugation

Product Specific Information

Description:
The Michel-17 monoclonal antibody reacts with mouse CD83, a 45 kDa cell surface glycoprotein and a member of the Ig superfamily. CD83 is predominantly expressed on mature dendritic cells (DCs) and activated lymphocytes. Cross-linking of CD83 with Michel-17 on DCs or activated T cells does not induce activation signals. CD83 plays an important role in T cell development through ligand interaction. CD83-Ig protein studies have shown a CD83 ligand mainly expressed by B220⁺ cells in mouse spleen.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Tested by flow cytometric analysis of LPS-activated mouse splenocytes.
• Recommended ≤0.5 µg per test (100 µL final volume).
• Optimal cell number: 10⁵–10⁸ cells/test (empirically determined).
• Antibody should be titrated for optimal assay performance.

Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD83 is a 40–45 kDa cell surface glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 when cultured with agents such as Con A or PHA. In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by cells within germinal centers. It is also expressed by Langerhans cells in the skin.

The CD83 antigen is a 186-amino-acid single-chain glycoprotein, part of the immunoglobulin superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane region, and a short cytoplasmic tail (~40 amino acids). CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of its core protein. CD83⁺ cells exhibit a unique surface phenotype distinct from T, B, NK, or myelomonocytic lineages, co-expressing high levels of MHC class II molecules and various lineage markers (CD2, CD5, CD40, CD78, CD13, CD33, CD36).

Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.