Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 615, 737
• Brilliant Violet 480, 605, 711, 786
• eFluor 450
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 645
• Custom conjugation available upon request

Product Specific Information

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. It binds strongly to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.

Applications Reported:

• Flow cytometric analysis
• Immunoprecipitation
• Immunohistology of frozen tissue sections
• Blocking of CD80-ligand interaction (Functional Grade recommended)

Applications Tested:
Validated by flow cytometric analysis of activated mouse splenocytes. Recommended ≤0.5 µg/test in 100 µL final volume. Cell count may vary (10⁵–10⁸ cells/test). Optimize titration for best performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells and is found in EBV-transformed, Burkitt’s lymphoma, follicular B lymphoma cells, T cells, and monocytes.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.