Thermo Fisher Scientific GST Monoclonal Antibody (3D4), DyLight 549
GST 단백질 검출용 마우스 단클론 항체(DyLight 549 결합형). Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 신호 대비 우수. 단기 4°C, 장기 -20°C 보관 권장. 연구용 한정 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D4 |
| Immunogen | BALB/c mice were immunized with GST from full-length Schistosoma japonicum protein. A hybridoma was produced by fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology. |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Available Formats
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and purified and partially purified Glutathione-S-Transferase (GST).
Target Information
Glutathione S-transferase (GST) is a 26 kDa protein present in both eukaryotes and prokaryotes, catalyzing a variety of reactions.
GST is widely used in the GST gene fusion system, allowing purification of fusion proteins via high affinity for glutathione.
Fusion proteins provide an important assay tool for studying direct protein-to-protein interactions.
The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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