Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), DyLight 488
GFP 단백질을 특이적으로 인식하는 DyLight 488 결합 단일클론 항체로, Western blot, ICC/IF, ELISA 등에 사용 가능. 높은 신호 대비와 낮은 배경을 제공하며, 연구용으로 안정적인 형광 검출이 가능함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F9.F9 |
| Immunogen | BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full-length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by fusing BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology. |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- AP
- Biotin
- DyLight 405
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- FITC
- HRP
- Rhodamine
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if the product is not completely clear after standing at room temperature. The product is stable for several weeks at 4°C as an undiluted liquid. Dilute only immediately before use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (Aequorea victoria) by Western blot. No cross-reactivity is seen against RFP.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in bioluminescence reactions. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic systems, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP enables localization studies, tracking of protein dynamics, and analysis of subcellular compartmentalization. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm). It is highly stable, species-independent, and suitable for various fluorescence-based applications such as FRET and yeast two-hybrid assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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