Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), DyLight 488
DyLight 488 형광으로 표지된 Phospho-AKT (Ser473) 단일클론 항체로, 인간 및 마우스 시료에서 AKT 인산화 상태 검출에 적합. Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용 가능. 안정적이며 고순도 Protein A 정제 제품.
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Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11), DyLight 488
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (IHC) | 20 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17F6.B11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein phosphorylated at S473.
BLAST analysis indicates cross-reactivity with AKT pS473 from human, mouse, rat, and chimpanzee (100% homology).
Cross-reactivity with AKT2 and AKT3 has not been determined.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. It inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets across cell types, including cancer cells.
Activated by insulin, PI3K, IGF1, and other growth factors, Akt promotes survival via phosphorylation and inactivation of forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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