Thermo Fisher Scientific CHKB Polyclonal Antibody
CHKB 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(P)에서 검증됨. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 단기 보관 또는 -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 300–342 of human ChoKB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553455 |
Product Specific Information
- Detects endogenous CHKB protein at ~45 kDa.
- Purity >95% by SDS-PAGE.
Target Information
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase, the initial enzyme in this sequence, plays a role in cell growth and proliferation.
ChoKB (choline kinase beta) is a 395 amino acid enzyme that catalyzes the phosphorylation of choline by ATP in the presence of magnesium, yielding phosphocholine and ADP.
Like all choline kinases, ChoKB also catalyzes the phosphorylation of ethanolamine.
The gene encoding ChoKB is located less than 1 kb upstream of the CPT1B gene, suggesting possible transcriptional regulation.
In mice, mutations or deletions in the ChoKB gene cause hindlimb muscular dystrophy and neonatal bone deformity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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