Thermo Fisher Scientific TCF3 Recombinant Rabbit Monoclonal Antibody (SY0258)
인간 TCF3 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 민감도를 제공. Western blot 및 유세포분석에 적합하며, 로트 간 일관성 및 동물 유래 성분이 없는 포뮬레이션. 장기 저장 시 -20°C 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Flow Cytometry (Flow)
- Tested Dilution: 1:50–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY0258 |
| Immunogen | Synthetic peptide within Human TCF3 aa 71–120 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809385 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
TCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, such as muscle or early B-cell differentiation.
Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) dimers bind DNA on E-box motifs (5'-CANNTG-3').
It binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases.
Two alternatively spliced human isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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