Thermo Fisher Scientific Phospho-Stat1 (Ser727) Recombinant Rabbit Monoclonal Antibody (Stat1S727-C6), FITC
인산화된 Stat1(Ser727)을 특이적으로 인식하는 FITC 결합 재조합 토끼 단클론 항체. Flow Cytometry에 적합하며 높은 특이성과 재현성을 제공. Protein A/G 정제, PBS 버퍼(0.2% BSA, 0.09% NaN₃) 포함, 4°C 암소 보관.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Stat1S727-C6 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897009 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
STAT1 (Signal Transducers and Activators of Transcription 1) is a member of the STAT family of transcription factors. It can be activated by interferon-α, interferon-γ, EGF, PDGF, and IL-6, regulating genes involved in cell growth, apoptosis, immune responses, and lipid metabolism.
STAT1 plays an important role in mediating cell viability in response to diverse stimuli and pathogens. Located on chromosome 2, STAT1 is activated via phosphorylation by JAK kinases and translocates to the nucleus to regulate gene transcription.
Among the seven STAT types, STAT1, STAT3, STAT5a, and STAT5b have broad activation profiles. STAT1 responds to various ligands including IFN family (IFN-α, IFN-β, IFN-γ, IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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