Thermo Fisher Scientific Phospho-Stat1 (Ser727) Recombinant Rabbit Monoclonal Antibody (Stat1S727-C6)
인간 STAT1 Ser727 인산화 부위를 인식하는 재조합 래빗 모노클로날 항체입니다. Western blot, ICC/IF, Flow cytometry에 적합하며 높은 특이성과 재현성을 제공합니다. 동물 유래 성분이 없는 포뮬레이션으로 연구용에 최적화되어 있습니다.
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Thermo Fisher Scientific Phospho-Stat1 (Ser727) Recombinant Rabbit Monoclonal Antibody (Stat1S727-C6)
Applications and Tested Dilution
- Western Blot (WB): Assay-dependent
- Immunocytochemistry (ICC/IF): 1:100
- Flow Cytometry (Flow): 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Stat1S727-C6 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897007 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
STAT1 (Signal Transducer and Activator of Transcription 1) is a transcription factor activated by interferons (α, β, γ), EGF, PDGF, and IL6. It regulates genes involved in cell growth, apoptosis, immune response, and lipid metabolism.
Located on chromosome 2, STAT1 activation occurs through phosphorylation by JAK kinases, followed by nuclear translocation to regulate gene transcription.
STAT1, STAT3, STAT5a, and STAT5b show wide activation profiles. STAT1 responds to multiple ligands including:
- IFN family (IFN-α, IFN-β, IFN-γ, IL-10)
- gp130 family (IL-6, IL-11, LIF, CNTF, G-CSF)
- Receptor tyrosine kinases (EGF, PDGF, CSF-1)
Two alternatively spliced transcript variants encoding distinct STAT1 isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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