Thermo Fisher Scientific TLE1 Monoclonal Antibody (OTI1A11), TrueMAB
TLE1 단백질을 인식하는 마우스 단클론 항체로 Western blot 및 IHC(P) 분석에 적합. 인간 시료 반응성. 동결건조 형태로 제공되며, PBS 완충액과 트레할로스 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 180–460 of human TLE1 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
TLE1 functions as a transcriptional corepressor that interacts with various transcription factors.
It inhibits NF-kappa-B-regulated gene expression and transcriptional activation mediated by FOXA2, CTNNB1, and TCF family members in Wnt signaling.
Its activity may be modulated through association with dominant-negative AES and may act as a coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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