Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Mouse Monoclonal Antibody (CR55T33), Alexa Fluor Plus 555

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• Request custom conjugation

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies utilize advanced dye chemistry for superior brightness and signal-to-noise ratio compared to conventional Alexa Fluor™ conjugates. As recombinant antibodies, they offer improved specificity and lot-to-lot consistency through controlled gene cloning and expression in vitro.

Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific background from potential aggregates.

Applications Tested:
This antibody (CR55T33) has been validated by Western blot and flow cytometry using Jurkat cells treated with 50 µM etoposide for 8 hours. Recommended starting concentrations:

• Western blot: 2 µg/mL (optimize per assay)

Optical Properties:

• Excitation: 553 nm
• Emission: 568 nm
• Laser: Yellow Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. Phosphorylation at serine 139 (γ-H2AX) marks sites of DNA double-strand breaks (DSBs) and can be detected by Western blot or immunofluorescence to assess DNA damage frequency.

Phosphorylation is mediated by phosphatidylinositol 3-kinases, potentially including ATM, DNA-PK, and ATR. H2A.X contains 143 amino acids and exists in two mRNA forms (~1600 bases with polyA and ~575 bases without). The gene maps to human chromosome 11q23.2–q23.3 and plays a key role in chromatin structure and DNA repair by recruiting MDC1 to damage sites.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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