Merck Anti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67

Anti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67

clone H1alpha67, Chemicon®, from mouse

HIF-1 alpha, ARNT Interacting Protein, MOP1

Quality Level

100

생물학적 소스

mouse

항체 형태

purified immunoglobulin

antibody product type

primary antibodies

클론

H1alpha67, monoclonal

species reactivity

mouse, rat, human, ferret

포장

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

electrophoretic mobility shift assay: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2b

NCBI 수납 번호

NM_001530.2
NM_181054.1

UniProt 수납 번호

Q16665

배송 상태

ambient

저장 온도

2-8°C

Gene Information

human ... HIF1A(3091)

HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis, the posttranslational modification by prolyl hydroxylation as a key regulatory event that targets HIF-alpha subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The transcriptional complex is composed of an alpha-beta heterodimer; HIF-beta being a constitutive nuclear protein that dimerises with oxygen regulated HIF-alpha subunits. In normoxia, 4-hydroxylation of human HIF-alpha at Pro402 or Pro564 by a set of HIF prolyl hydroxylase isoenzymes (PHD 1-3) mediates HIF1-alpha recognition by von Hippel-Lindau ubiquitin ligase complex leading to its proteasomal destruction. In hypoxia (deprivation of oxygen), lack of hydroxylase activity enables HIF-alpha subunits to escape destruction and become transcriptionally active. Thus HIF hydroxylases provide a focus for understanding cellular responses to hypoxia and target for therapeutic manipulation. There are several HIF factors, which include HIF 1-alpha, HIF 1-beta, HIF 2-alpha. HIF 1-alpha is an 812 a.a. protein in rat and 836 a.a. long in mouse and human. A master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. It is ubiquitous in expression as cytoplasmic in normoxia, nuclear translocation in response to hypoxia.