Merck Anti-Podocin, Mouse monoclonal

Anti-Podocin, Mouse Monoclonal

Clone: PD-65
Purified from hybridoma cell culture

Target: NPHS2 (Podocin)

Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes interdigitate to form filtration slits bridged by a thin slit diaphragm. This adherens-like junction acts as a barrier to macromolecules and plays a crucial role in glomerular filtration.

Podocin (NPHS2 protein) is a key component of the slit diaphragm complex. It belongs to the band-7-stomatin family of lipid raft-associated proteins and is a hairpin-like integral membrane protein with intracellular N- and C-termini.

Podocin is located at the insertion site of the slit membrane and functions as a scaffold protein maintaining the structural integrity of the slit diaphragm. It interacts with nephrin (NPHS1 protein) and the adapter protein CD2-AP, both essential for kidney filtration barrier function.

Podocin is anchored in glycosphingolipid- and cholesterol-rich lipid rafts of the plasma membrane and partially associates with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling via AP-1 in HEK cells. Beyond the kidney, podocin expression has been reported in rat nervous tissue and possibly in vascular smooth muscle.

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