Merck Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4

Anti-Glycosaminoglycan Antibody, Skin Specific Antibody, Clone PG-4

Clone PG-4 (from Mouse)

Proteoglycan, Dermatan Sulfate, Chondroitin Sulfate

Proteoglycans (PG) are matrix molecules found in all tissues and are among the most studied components of the extracellular matrix. Over 50 PG members have been characterized for their biological significance. Each PG consists of a protein core covalently linked to glycosaminoglycan (GAG) chains.

GAGs are natural heteropolysaccharides composed of repeating disaccharide units, which may be sulfated or non-sulfated. Their molecular size and sulfation patterns vary by tissue type. Major GAG types in mammalian tissues include:

• Hyaluronan
• Chondroitin sulfate
• Dermatan sulfate
• Heparin and heparan sulfate
• Keratan sulfate

GAG chains are covalently attached to serine residues of the PG protein core via a tetrasaccharide linkage (xylose–two galactose residues–glucuronic acid).

This antibody, clone PG-4, recognizes antigens associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and adult keratinocytes. It binds native epitopes within glycosaminoglycan chains and detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin, Biglycan) proteoglycans.

Treatment of proteoglycans with chondroitinases abolishes immunoreactivity for all antigens.

참고 문헌

• Afratis, N. et al. (2012). FEBS Journal, 279(7): 1177–1197
• Sorrell, J.M. et al. (1999). Histochem. J., 31(8): 549–558

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