Merck Anti-GAPDH Antibody

Anti-GAPDH Antibody

from chicken, purified by affinity chromatography

Target Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a "house-keeping" enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a high-energy intermediate used for the synthesis of ATP.

Besides its cytoplasmic role in metabolism, GAPDH is also involved in the initial stages of apoptosis and oxidative stress response, where it translocates to the nucleus. This nuclear localization may reflect its role in DNA repair or as a carrier for apoptotic molecules.

GAPDH binds specifically to proteins implicated in neurodegenerative disorders, including beta-amyloid precursor protein and huntingtin protein. Decreased GAPDH function is associated with Alzheimer’s and Huntington’s disease fibroblasts. It is also identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

The complete and functional enzyme is a tetramer, with each of four identical subunits forming a tetrahedral structure. Binding domains include one interacting with NAD+ and another with glyceraldehyde 3-phosphate (GAP).

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