Merck SUMO Protease, Biotin tagged

SUMO Protease, Biotin tagged

Recombinant protein, aqueous solution, ≥25,000 units/mL

Small Ubiquitin-like Modifier Protease, ULP, Ubiquitin-homology domain protein PIC1, Ubiquitin like protease, Ubl-specific protease 1

재조합

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

분자량

27 kDa

농도

≥25,000 units/mL

배송 상태

dry ice

저장 온도

−20°C

SUMO proteases are enzymes that specifically cleave the post-translational protein modification (PTM) known as small ubiquitin-related modifier (SUMO). SUMO falls into the PTM class of ubiquitin and/or ubiquitin-like proteins (UBL).SUMO protease is the Ubl-specific protease 1 (Ulp1) from Saccharomyces cerevisiae. This was the first of this class of enzymes to be isolated. SUMO protease cleaves specifically the SUMO moiety in a ‘scarless′ manner. After recognizing the tertiary structure of the Ubiquitin-like SUMO domain, SUMO protease hydrolyzes the peptide bond in the x–Gly–Gly–x sequence after the Gly-Gly bond, at the C-terminus of the SUMO domain. Besides the cleavage of natural SUMO-modified proteins, SUMO protease is used to cleave recombinant SUMO fusion proteins. The SUMO domain is a known solubility-enhancing fusion tag used in recombinant protein expression. Since this recombinant protease does not contain any coman protein purification tag it can be used for on-column cleavage of column bound SUMO fusion protein. Sumo protease with Biotin tag can be easily removed at the end of the digestion reaction.