| 상품 옵션 정보 | ||||||||
|---|---|---|---|---|---|---|---|---|
| 카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
| DUO82047-20L | - | Merck DUO82047-20L Duolink In Situ Wash Buffer, Brightfield, 20 L pk | 재고문의 | pk | 538,440원 | - | 592,284원 | |
| DUO82047-4L | - | Merck DUO82047-4L Duolink In Situ Wash Buffer, Brightfield, 4 L pk | 재고문의 | pk | 137,340원 | - | 151,074원 | |
Duolink® In Situ Wash Buffer, Brightfield
Quality Level
material
packing (powdered buffer pouches)
제품 라인
Duolink®
technique(s)
proximity ligation assay: suitable
적합성
suitable for brightfield
저장 온도
20-25°C
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the [Duolink® In Situ Brightfield Protocol to use this product.
Visit our](/technical-documents/protocols/biology/duolink-brightfield-user-manual.html) Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using HRP for brightfield detection.
Specificity
Duolink® In Situ Wash Buffer, Brightfield is used to wash specimen slides during Duolink® In Situ Brightfield procedures. See datasheet for more details.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
배송/결제/교환/반품 안내
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