Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

Target microRNA

mmu-miR-1934-3p

Design

Tough Decoy (TuD)

제품 라인

MISSION®

품질 등급

200

물리적 형태

liquid

농도

≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

AGGAUGACGGUGGGGCUGGUGA

Sanger 성숙/소수 수납 번호

MIMAT0017341

microRNA 수납 번호

MI0009923

배송 상태

dry ice

저장 온도

−70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design. miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles suitable for mammalian cell transduction.
Includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) to enhance transgene expression.
The vector carries a puromycin resistance gene for selection of transduced cells.

주요 특징

• Potent inhibition of target miRNA
• Lentiviral delivery enables efficient transduction into diverse cell types
• Long-term inhibition without the need for repeat transfection