Merck MISSION Lentiviral Packaging Mix

MISSION® Lentiviral Packaging Mix

Production of replication incompetent viral particles

Quality Level

200

제품 라인

MISSION®

저장 온도

−20°C

The MISSION® shRNA product line is centered on a transfer vector-based RNAi library against annotated mouse and human genes that can be used in conjunction with this Lentiviral Packaging Mix. In this packaging system, the pseudo-typing with VSV-G broadens the viral tropism associated with the virus. Therefore, these lentiviral particles can efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells.

The MISSION® Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Lentiviral particles are generated from three main components:

• The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
• The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping.
• The shRNA transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for RNA production and packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications.