Merck AccuChIP Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control

AccuChIP Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control

Target-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:

• Normalization of ChIP signals

• Normalization of ChIP-seq signals

• Analysis of antibody specificity

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable

배송 상태

dry ice

AccuChIP Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments.

Features and Advantages

• Simple to use - just add to chromatin either before or after sonication
• Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
• Target specific control - uses the same antibody used for your ChIP experiment
• Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
• Easily incorporated into virtually any ChIP protocol
• Readily detected by qPCR
• Easily incorporated into ChIP-seq libraries
• Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity