Merck PSF-TEFI-TPI1-RLUC-URA3 - RENILLA LUCIFERASE YEAST PLASMID

PSF-TEFI-TPI1-RLUC-URA3 - RENILLA LUCIFERASE YEAST PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 8512 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

Renilla luciferase

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

A renilla luciferase Saccharomyces cerevisiae yeast plasmid that allows the co-expression of a gene of interest alongside the luciferase reporter. The vector also contains the URA3 selection cassette that allows the plasmid to be grown in cells that are defective for this gene when they are grown in uracil deficient media.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the expression of the reporter gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).