Merck PSF-MINCMV-RLUC - MINIMAL CMV PROMOTER RENILLA PLASMID

PSF-MINCMV-RLUC - MINIMAL CMV PROMOTER RENILLA PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4669 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

Renilla luciferase

배송 상태

ambient

저장 온도

−20°C

In this plasmid the Renilla luciferase gene is under transcriptional regulation of the minimal CMV promoter. This system gives only low level expression in most mammalian cells however an MCS upstream of the minimal promoter can be used to insert positive- or negative- regulatory elements for example to introduce responsiveness to cell-associated transcription factors or exogenously-added drugs. In this way a range of reporter systems can be prepared.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.