Merck PSF-TEFI-TPI1-NEO/G418 - G418 YEAST SELECTION PLASMID

PSF-TEFI-TPI1-NEO/G418 - G418 YEAST SELECTION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 7252 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This is a yeast expression plasmid containing the strong TEF1 constitutive promoter that allows for the expression of transgenes in Saccharomyces cerevisiae. The vector also contains a resistance gene for G418 and allows plasmids to be selected and grown in yeast cells using this antibiotic.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).