Merck PSF-TEFI-TPI1-ZEO - ZEOCIN YEAST SELECTION VECTOR

PSF-TEFI-TPI1-ZEO - ZEOCIN YEAST SELECTION VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6832 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid can be used for the expression of transgenes in Saccharomyces cerevisiae under the control of the constitutive TEF1 yeast promoter. The vector also contains a resistance gene that allows for cells containing the plasmid to be selected using the antibiotic zeocin. The vector contains our standard multiple cloning site that allows you to clone in a transgene of interest.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).