Merck PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID

PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3858 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: OXB18
Promoter activity: constitutive
Promoter type: bacterial

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-OXB18 - VERY STRONG E.COLI PROMOTER PLASMID that allows the production of protein in bacteria. The plasmid contains our standard multiple cloning site downstream of the OXB18 promoter. We developed this promoter in house by modifying the RecA E. coli promoter so that it no longer repressed by LexA and also incorporates a series of mutations that make it slightly weaker. This is part of an extended product range of bacterial promoters with different transcriptional activities. OXB20 is the strongest in this range and OXB1 is the weakest. This plasmid vector does not require an inducing agent for activity.

Promoter Expression Level: This molecular cloning vector contains a very strong constitutive E. coli promoter that was derived from the RecA promoter by random mutagenesis. It is part of our constitutive bacterial promoter range. This promoter (OXB18) shows the very high levels of expression in the range with OXB1 showing the lowest level and OXB20 showing the highest level of expression. This vector require no inducing agent for expression.