Merck PSF-CAG-AMP - CAG PROMOTER PLASMID

PSF-CAG-AMP - CAG PROMOTER PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5431 bp

박테리아 선정

ampicillin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CAG
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This mammalian expression vector contains the chimeric CAG promoter which is a composite of the CMV enhancer the chicken beta actin promoter (CBA) and the rabbit beta globin intron. This promoter is often used instead of the CMV promoter because in some cell types the CMV promoter can get silenced. To prevent this the CAG promoter contains the CpG island from the CBA promoter to help prevent promoter methylation. The plasmid can be used to drive protein expression in a range of mammalian cell types and we have consistently found that expression levels are equal to CMV in short term experiments in standard cell types.

Promoter Expression Level: The plasmid vector contains the mammalian CAG promoter which is a synthetic composite of the CMV immediate early enhancer followed by the CBA promoter and the rabbit beta globin intron. The Chicken beta actin contains a CpG island that can help to keep the promoter active for longer in stable culture when compared to the CMV promoter.